Device

Part:BBa_K1613006:Design

Designed by: Ko-yu Chang   Group: iGEM15_HSNU-TAIPEI   (2015-09-05)


A composite part that can detect some toxins.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 65
    Illegal suffix found in sequence at 1654
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 65
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 1655
    Illegal PstI site found at 1669
    Illegal NotI site found at 71
    Illegal NotI site found at 1662
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 65
    Illegal BglII site found at 1484
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 65
    Illegal suffix found in sequence at 1655
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 65
    Illegal XbaI site found at 80
    Illegal SpeI site found at 1655
    Illegal PstI site found at 1669
    Illegal NgoMIV site found at 404
    Illegal NgoMIV site found at 491
    Illegal AgeI site found at 474
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1204


Design Notes

use cyp1a2 to detect some toxins like aflatoxin B1.


Source

We use humen liver gene cyp1a2(genebank:NM_000761),but we do some chang to remove the restriction site.

References